Talking about the quality control of the results of the automatic blood cell analyzer

At present, automatic blood cell analyzer has been very popular in the blood routine test in various laboratories, which greatly improves the efficiency of blood cell analysis. However, due to the variety of instruments, the performance of large differences, and the working principles of various instruments are not the same, the completion of a project determination by instruments, reagents, calibrations, operating procedures and operators and other factors. Therefore, how to avoid the difference between the results of different instruments, how to improve the accuracy of the results of the blood analyzer, comprehensive quality control is a very important work. The author of the monitor manufacturer outlines the following aspects:

1. Requirements for the quality of operators

Man is the first element, the operator of the experimental apparatus, and must undergo specialized technical training. Understand the structure of each part of the instrument, familiar with the performance of the instrument, working principle, parameter settings, daily maintenance and reagent application. It has the ability to explain and process all kinds of alarm information of the instrument, and eliminate the general trouble shooting of the instrument.

2. The performance of the hematology analyzer is evaluated.

Before the new instrument is used for clinical measurement after installation and debugging, the performance indexes of the new instrument must be evaluated preliminarily. In accordance with the relevant provisions of the International Commission for Standardization of Hematology (ICSH) and the American Institute of Clinical Laboratory Standardization (CLSI), the performance indexes of the instrument were evaluated, including the linear range, precision, accuracy, anti-interference, white blood cell classification and carrying contamination rate.  Conditions should be compared with other instruments with stable performance, good operation and traceable results. In this way, we can have a comprehensive understanding of the actual performance of the new instrument.

3. Create a good operating environment for automatic blood cell analyzer.

The normal operation of the automatic blood cell analyzer must rely on a good operating environment. It includes a power supply system with stable current, voltage and ground protection. The test bench should be stable and sunlight-proof. The laboratory should be shock-proof, dust-proof, moisture-proof and ventilated. The indoor temperature should be 15 ~25 ~C, and the relative humidity should be less than 80%. The instrument should be located far away from the electromagnetic interference source and the heat source. It has a relatively independent running environment. Any changes in environmental factors may affect the stability of the instrument, thereby affecting the accuracy and accuracy of the results of measurement. Therefore, we should always observe and do a good job of corresponding records, timely correction.

4. Correct calibration of automatic blood cell analyzer.

The calibration of the automatic blood cell analyzer directly affects the correctness of the test results. Corresponding calibration methods can be adopted: (1) the matching blood cell calibration products provided by the instrument manufacturer should be selected for calibration, which is the most ideal and practical method, but the original calibration products are relatively expensive and have a short validity period and are not easy to popularize; (2) fresh whole blood samples for calibration, that is, fresh blood samples have been used in other already. Determine to use a complete set of calibration products calibrated and stable performance of the automatic blood cell analyzer for testing, measuring the mean value of many times, will be determined as the standard value of the specimen, to calibrate the instrument to be calibrated, this will also get a more satisfactory result, can also be used in this way to calibrate the same laboratory different Automatic blood cell analyzer ensures the consistency of the results of different automatic blood cell analyzers in our laboratory.

5, strengthen the quality requirements for reagents.

Different manufacturers and different models of automatic blood cell analyzers have different requirements for reagents. At present, there are many kinds of reagents, such as imported matching, domestic and laboratory-made, etc. Different reagents, even if the formulation of the reagent is the same, some intrinsic parameters are not the same, mainly including the reagent pH, conductivity, osmotic pressure and ionic strength. The automatic blood cell analyzer is very sensitive to the subtle changes of the above parameters, especially in leukocyte classification. Therefore, we suggest it is better to use the instrument to match the original reagent to ensure the accuracy of the result.Meilun

6. Quality control before blood specimen analysis.

There are many links in blood samples before analysis, which are performed by doctors, nurses, nurses and laboratory instrument operators. During the collection process, the laboratory can not directly supervise and interfere with blood samples, and is the most prone to make mistakes. Therefore, we should take the initiative to communicate with the staff concerned, such as blood collection requirements, such as the amount of blood, what kind of anticoagulant tube, how to mix, as well as the inspection time limit and matters needing attention. When blood samples are received, attention should be paid to whether the specimen is contaminated, hemolysis, small clots or fibrous filaments and other conditions, the problematic specimen should be re-collected. It should be noted in the report sheet that jaundice, lipids and blood may affect the results of the determination, suggesting that the clinician.Meilun

7, indoor and indoor quality evaluation requirements

Quality control is based on the ISO9000:2000 document "quality management system" to determine the indicators and results of quality control products. Indoor quality control mainly controls the stability of instruments, which is one of the means to control the correctness of instruments. Therefore, every day the instrument is randomized to do quality control and strictly comply with the relevant requirements of the quality control specification. Such as the use of quality control products, storage conditions and matters needing attention, and faithfully report the results of determination, draw quality control chart. Analysis and Research on indoor quality control within a certain period of time, once beyond the scope of quality control, we should actively find out the reasons, take effective measures, and constantly correct the work.